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Issue 15, 2019
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A cell-permeant small molecule for the super-resolution imaging of the endoplasmic reticulum in live cells

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Abstract

The endoplasmic reticulum (ER) constitutes about half of the total membrane of a eukaryotic cell, and defects in the ER have been shown to be linked with a variety of diseases. To investigate these underlying mechanisms in detail, the specific labelling of the ER for high-resolution long-term live-imaging can serve as an important tool. Here, we report the identification of a stimulated emission depletion (STED) microscopy-compatible BODIPY derivative (NH2-BODIPY) to selectively image the ER. In contrast to the conventional ER-Tracker™ dye, NH2-BODIPY selectively labels the ER at a much lower concentration with no detectable cytotoxicity. Conventional imaging agents are often unstable under the intense light field used for STED microscopy, but NH2-BODIPY is stable due to its robust structure and therefore it has the potential to be widely exploited for ER imaging.

Graphical abstract: A cell-permeant small molecule for the super-resolution imaging of the endoplasmic reticulum in live cells

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Supplementary files

Article information


Submitted
19 Feb 2019
Accepted
19 Mar 2019
First published
19 Mar 2019

Org. Biomol. Chem., 2019,17, 3732-3736
Article type
Communication

A cell-permeant small molecule for the super-resolution imaging of the endoplasmic reticulum in live cells

A. R. Sekhar, B. Mallik, V. Kumar and J. Sankar, Org. Biomol. Chem., 2019, 17, 3732
DOI: 10.1039/C9OB00408D

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