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Issue 23, 2019
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Experimental and numerical evaluation of a genetically engineered M13 bacteriophage with high sensitivity and selectivity for 2,4,6-trinitrotoluene

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Abstract

Selective and sensitive detection of desired targets is very critical in sensor design. Here, we report a genetically engineered M13 bacteriophage-based sensor system evaluated by quantum mechanics (QM) calculations. Phage display is a facile way to develop the desired peptide sequences, but the resulting sequences can be imperfect peptides for binding of target molecules. A TNT binding peptide (WHW) carrying phage was self-assembled to fabricate thin films and tested for the sensitive and selective surface plasmon resonance-based detection of TNT molecules at the 500 femtomole level. SPR studies performed with the WHW peptide and control peptides (WAW, WHA, AHW) were well-matched with those of the QM calculations. Our combined method between phage engineering and QM calculation will significantly enhance our ability to design selective and sensitive sensors.

Graphical abstract: Experimental and numerical evaluation of a genetically engineered M13 bacteriophage with high sensitivity and selectivity for 2,4,6-trinitrotoluene

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Publication details

The article was received on 11 Dec 2018, accepted on 18 Mar 2019 and first published on 27 Mar 2019


Article type: Communication
DOI: 10.1039/C8OB03075H
Org. Biomol. Chem., 2019,17, 5666-5670

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    Experimental and numerical evaluation of a genetically engineered M13 bacteriophage with high sensitivity and selectivity for 2,4,6-trinitrotoluene

    W. Kim, C. Zueger, C. Kim, W. Wong, V. Devaraj, H. Yoo, S. Hwang, J. Oh and S. Lee, Org. Biomol. Chem., 2019, 17, 5666
    DOI: 10.1039/C8OB03075H

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