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Issue 8, 2019
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DNA triplex-based fluorescence turn-on sensors for adenosine using a fluorescent molecular rotor 5-(3-methylbenzofuran-2-yl) deoxyuridine

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Abstract

Fluorescence turn-on sensors for adenosine were developed using DNA triplexes modified with a fluorescent molecular rotor 5-(3-methylbenzofuran-2-yl)deoxyuridine (dUMBF) and abasic sites. Binding of adenosine to the abasic site next to the dUMBF changed the microenvironment and conformation (from the twisted to planar state) of dUMBF and enhanced the fluorescence. Adenosine could be selectively detected over other nucleosides and adenosine phosphates. The binding of adenosine was confirmed by UV-thermal melting experiments. Further, the conformational changes of dUMBF from the twisted to coplanar state upon binding of adenosine was supported by MD simulations.

Graphical abstract: DNA triplex-based fluorescence turn-on sensors for adenosine using a fluorescent molecular rotor 5-(3-methylbenzofuran-2-yl) deoxyuridine

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Publication details

The article was received on 06 Nov 2018, accepted on 07 Jan 2019 and first published on 25 Jan 2019


Article type: Communication
DOI: 10.1039/C8OB02747A
Org. Biomol. Chem., 2019,17, 2077-2080

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    DNA triplex-based fluorescence turn-on sensors for adenosine using a fluorescent molecular rotor 5-(3-methylbenzofuran-2-yl) deoxyuridine

    T. Kanamori, Y. Masaki, Y. Oda, H. Ohzeki, A. Ohkubo, M. Sekine and K. Seio, Org. Biomol. Chem., 2019, 17, 2077
    DOI: 10.1039/C8OB02747A

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