A simple fluorescence aptasensor for gastric cancer exosome detection based on branched rolling circle amplification

Abstract

Exosomes are membrane nanovesicles carrying molecular information that may reflect the biological and genetic characteristics of original cells. Numerous studies have demonstrated the potential of exosomes as noninvasive cancer biomarkers. Hence, specific detection of cancer cell-derived exosomes is of significant importance. Here we developed a fluorescence assay for the determination of gastric cancer exosomes based on branched rolling circle amplification (BRCA) and an aptamer specific to target exosome. The designed padlock probe was cyclized after incubation with aptamer binding with target exosome. BRCA was triggered by adding a second primer and the resulting long tandem double-strand DNA product was detected using SYBR Green I as the fluorescent dye. This method demonstrated a high specificity for target exosomes with a detection limit of 4.27×104 exosomes/mL. Moreover, plasma from gastric cancer patients was tested to verify the clinical applicability of this assay. Our results demonstrated that this aptamer-based biosensor may show potential for the early diagnosis of gastric cancer.