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An RNase H-powered DNA walking machine for sensitive detection of RNase H and the screening of related inhibitors

Abstract

Ribonuclease H (RNase H), an intracellular ribonuclease, plays a crucial role in cellular processes, and especially relates with many diseases processes. Here, we report a novel signal amplification strategy based on an RNase H-powered DNA walking machine for specific and sensitive RNase H activity detection. The DNA walking machine is composed of a small quantity DNA walker strands and abundant FAM-labeled DNA-RNA chimeric strands on a single gold nanoparticle (AuNP). In the presence of the RNase H, the RNase H can specifically degrade the RNA fragment in DNA-RNA hybrid duplex and trigger the autonomous movement of DNA walker strand on the AuNP surface. During this process, each step of the walking can release the FAM-labeled RNA from the surface of AuNP, realizing the signal amplification for RNase H sensing. This method has been successfully utilized for the RNase H activity detection in the complex system and applied for the screening of related inhibitors. Therefore, our RNase H-powered DNA walking machine gives a novel platform for the RNase H activity detection and the RNase H-associated drug discovery.

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Supplementary files

Publication details

The article was received on 01 Sep 2019, accepted on 03 Dec 2019 and first published on 04 Dec 2019


Article type: Paper
DOI: 10.1039/C9NR07550J
Nanoscale, 2019, Accepted Manuscript

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    An RNase H-powered DNA walking machine for sensitive detection of RNase H and the screening of related inhibitors

    Y. Wang, N. Hu, C. Liu, C. Nie, M. He, J. Zhang, Q. Yu, C. Zhao, T. Chen and X. Chu, Nanoscale, 2019, Accepted Manuscript , DOI: 10.1039/C9NR07550J

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