Issue 45, 2019

Investigating the origin of high efficiency in confined multienzyme catalysis

Abstract

Biomimetic strategies have successfully been applied to confine multiple enzymes on scaffolds to obtain higher catalytic efficiency of enzyme cascades than freely distributed enzymes. However, the origin of high efficiency is poorly understood. We developed a coarse-grained, particle-based model to understand the origin of high efficiency. We found that a reaction intermediate is the key in affecting reaction kinetics. In the case of unstable intermediates, the confinement of multiple enzymes in clusters enhanced the catalytic efficiency and a shorter distance between enzymes resulted in a higher reaction rate and yield. This understanding was verified by co-encapsulating multiple enzymes in metal–organic framework (MOF) nanocrystals as artificially confined multienzyme complexes. The activity enhancement of multiple enzymes in MOFs depended on the distance between enzymes, when the decay of intermediates existed. The finding of this study is useful for designing in vitro synthetic biology systems based on artificial multienzyme complexes.

Graphical abstract: Investigating the origin of high efficiency in confined multienzyme catalysis

Supplementary files

Article information

Article type
Paper
Submitted
27 Aug 2019
Accepted
15 Oct 2019
First published
16 Oct 2019

Nanoscale, 2019,11, 22108-22117

Investigating the origin of high efficiency in confined multienzyme catalysis

Y. Cao, X. Li, J. Xiong, L. Wang, L. Yan and J. Ge, Nanoscale, 2019, 11, 22108 DOI: 10.1039/C9NR07381G

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