High-Throughput Single Nanoparticle Detection Using a Feed-Through Channel-Integrated Nanopore
Outstanding sensitivity of solid-state nanopore sensors comes at a price of low detection efficiency due to a lack of active means to transfer objects into the nanoscale sensing zone. Here we report on a key technology for high-throughput single-nanoparticle detections that exploits mutual effects of microfluidics control and multipore electrophoresis in nanopore-in-channel units integrated on a thin Si3N4 membrane. Using this novel nanostructure, we demonstrated a proof-of-concept for influenza viruses via hydropressure regulation of mass transport in the fluidic channel for continuous feeding of biosamples into the effective electric field extending out from the nanopores, wherein the feed-through mechanism allowed to selectively detect charged objects in physiological media such as human saliva. With the versatility of nanopore sensing technologies applicable to analytes of virtually any sizes from cell to polynucleotide, the present integration strategy may open new venue for practical ultrasensitive bioanalytical tools.