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Fluorescent polymer dots and graphene oxide based nanocomplex for “off-on” detection of metalloproteinase-9

Abstract

Numerous studies have demonstrated that cancer-related matrix metalloproteinase-9 (MMP-9) is an ideal biomarker for cancer diagnosis. However, most MMP-9 detection methods are expensive and time-consuming, and more convenient and specific MMP-9 detection methods are needed both clinically and in research. In the present study, peptide-linked polymer dots were assembled onto a graphene oxide surface to construct a graphene oxide-peptide-polymer dots (GO-Pep-Pdots) nanocomplex for sensitive, rapid, and accurate detection of MMP-9. In the absence of MMP-9, the nanocomplex was in an “off” state, whereas in the presence of MMP-9, the nanocomplex was turned “on”, resulting in the emission of a fluores-cence signal that linearly correlated with MMP-9 concentration. The limit of detection of the nanocomplex was 3.75 ng/mL, lower than most methods. This method was successfully verified by detecting MMP-9 in clinical serum samples of prostate cancer. The results suggest that this protease nanocomplex is generic and can be adopted to respond to other proteases by selecting specific peptides with suitable cleavage sites in clinic.

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Supplementary files

Publication details

The article was received on 31 Jul 2019, accepted on 19 Sep 2019 and first published on 11 Oct 2019


Article type: Paper
DOI: 10.1039/C9NR06557A
Nanoscale, 2019, Accepted Manuscript

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    Fluorescent polymer dots and graphene oxide based nanocomplex for “off-on” detection of metalloproteinase-9

    Q. Li, Y. Wang, G. Yu, Y. Liu, K. Tang, C. Ding, H. Chen and S. Yu, Nanoscale, 2019, Accepted Manuscript , DOI: 10.1039/C9NR06557A

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