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Issue 39, 2019
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Optical super-resolution microscopy unravels the molecular composition of functional protein complexes

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Abstract

Optical super-resolution microscopy has revolutionized our understanding of cell biology. Next to visualizing cellular structures with near-molecular spatial resolution, an additional benefit is the molecular characterization of biomolecular complexes directly in an intact cell. Single-molecule localization microscopy, as one technology out of the toolbox of super-resolution methods, generates images by detecting the position of single fluorophore labels and is particularly suited for molecular quantification. We review imaging and analysis methods employing single-molecule localization microscopy and extract molecule numbers.

Graphical abstract: Optical super-resolution microscopy unravels the molecular composition of functional protein complexes

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Article information


Submitted
25 Jul 2019
Accepted
23 Sep 2019
First published
26 Sep 2019

Nanoscale, 2019,11, 17981-17991
Article type
Review Article

Optical super-resolution microscopy unravels the molecular composition of functional protein complexes

M. S. Dietz and M. Heilemann, Nanoscale, 2019, 11, 17981
DOI: 10.1039/C9NR06364A

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