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Issue 35, 2019
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Single molecule protein patterning using hole mask colloidal lithography

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Abstract

The ability to manipulate single protein molecules on a surface is useful for interfacing biology with many types of devices in optics, catalysis, bioengineering, and biosensing. Control of distance, orientation, and activity at the single molecule level will allow for the production of on-chip devices with increased biological activity. Cost effective methodologies for single molecule protein patterning with tunable pattern density and scalable coverage area remain a challenge. Herein, Hole Mask Colloidal Lithography is presented as a bench-top colloidal lithography technique that enables a glass coverslip to be patterned with functional streptavidin protein onto patches from 15–200 nm in diameter with variable pitch. Atomic force microscopy (AFM) was used to characterize the size of the patterned features on the glass surface. Additionally, single-molecule fluorescence microscopy was used to demonstrate the tunable pattern density, measure binding controls, and confirm patterned single molecules of functional streptavidin.

Graphical abstract: Single molecule protein patterning using hole mask colloidal lithography

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Publication details

The article was received on 04 Jul 2019, accepted on 15 Aug 2019 and first published on 27 Aug 2019


Article type: Communication
DOI: 10.1039/C9NR05630K
Nanoscale, 2019,11, 16228-16234

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    Single molecule protein patterning using hole mask colloidal lithography

    W. Lum, D. Gautam, J. Chen and L. B. Sagle, Nanoscale, 2019, 11, 16228
    DOI: 10.1039/C9NR05630K

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