Large-scale rapid detection of circulating microRNAs in plasma for diagnosis and screening of specific diseases
microRNAs are a type of evolutionarily conserved small non-coding RNAs with a length of 18-25 nucleotides. In recent years, increasing studies have shown that the content of specific miRNAs in the blood changes significantly during the occurrence and development of major diseases such as cardiovascular disease and cancer. Therefore, miRNAs may serve as important new biomarkers that can be used for disease diagnosis in the future. Here, we improved the polyethylene glycol layer on the surface of traditional silicon sphere to specifically capture miRNAs by means of a full-function microplate detector, at 100 micros. The detection limit for specific miRNAs in liter plasma can reach 1 fM, and simultaneous detection of 96 samples can be achieved. Compared with the traditional real-time PCR technology, our detection eliminates the cumbersome environment of miRNA extraction, reverse transcription, amplification, etc., and avoids more human error in the detection process. Together with the full-featured microwell detector, we can rapidly detect specific miRNAs in plasma, which can be used in the diagnosis of cardiovascular diseases in the future.