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Issue 44, 2019
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Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes

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Abstract

There is a critical need for the development of safe and efficient delivery technologies for CRISPR/Cas9 to advance translation of genome editing to the clinic. Non-viral methods that are simple, efficient, and completely based on biologically-derived materials could offer such potential. Here we report a simple and modular tandem peptide-based nanocomplex system with cell-targeting capacity that efficiently combines guide RNA (sgRNA) with Cas9 protein, and facilitates internalization of sgRNA/Cas9 ribonucleoprotein complexes to yield robust genome editing across multiple cell lines.

Graphical abstract: Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes

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Publication details

The article was received on 27 Feb 2019, accepted on 15 Oct 2019 and first published on 29 Oct 2019


Article type: Paper
DOI: 10.1039/C9NR01786K
Nanoscale, 2019,11, 21317-21323
  • Open access: Creative Commons BY-NC license
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    Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes

    P. K. Jain, J. H. Lo, S. Rananaware, M. Downing, A. Panda, M. Tai, S. Raghavan, H. E. Fleming and S. N. Bhatia, Nanoscale, 2019, 11, 21317
    DOI: 10.1039/C9NR01786K

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