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Amplification-free SERS analysis of DNA mutation in cancer cells with single-base sensitivity

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Abstract

Accurate and sensitive identification of DNA mutations in tumor cells is critical to the diagnosis, prognosis and personalized therapy of cancer. Conventional polymerase chain reaction (PCR)-based methods are limited by the complicated amplification process. Herein, an amplification-free surface enhanced Raman spectroscopy (SERS) approach which directly detects point mutations in cancer cells has been proposed. A highly sensitive and uniform SERS substrate was fabricated using gold@silver core–shell nanorods, achieving an enhancement factor of 1.85 × 106. By combining the SERS-active nanosubstrate with molecular beacon probes, the limit of detection reached as low as 50 fM. To enable parallel analysis and automated operation, the SERS sensor was integrated into a microfluidic chip. This novel chip-based assay was able to differentiate between mutated and wild-type KRAS genes among a variety of other nucleic acids from cancer cells in 40 min. Owing to the simple operation and fast analysis, the SERS-based DNA assay chip could potentially provide insights into clinical cancer theranostics in an easy and inexpensive manner at the point of care.

Graphical abstract: Amplification-free SERS analysis of DNA mutation in cancer cells with single-base sensitivity

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Publication details

The article was received on 16 Jan 2019, accepted on 29 Mar 2019 and first published on 01 Apr 2019


Article type: Paper
DOI: 10.1039/C9NR00501C
Citation: Nanoscale, 2019, Advance Article

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    Amplification-free SERS analysis of DNA mutation in cancer cells with single-base sensitivity

    L. Wu, A. Garrido-Maestu, J. R. L. Guerreiro, S. Carvalho, S. Abalde-Cela, M. Prado and L. Diéguez, Nanoscale, 2019, Advance Article , DOI: 10.1039/C9NR00501C

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