Issue 14, 2019

Dual responsive specifically labelled carbogenic fluorescent nanodots for super resolution and electron microscopy

Abstract

Due to their high biocompatibility and nontoxic nature, carbogenic fluorescent nanodots (FNDs) have already shown their application in bioimaging. However, their non-specific labeling has restricted their application in live cell super resolution microscopy (SRM). Here we introduce, for the first time, an orange emissive FND, specifically conjugated to the HeLa cell actin filament, for successful single molecule stochastic optical reconstruction microscopy (STORM) and super resolution radial fluctuation (SRRF) microscopy. The resolution obtained in SRRF (∼35 nm) was almost an order of magnitude less than the diffraction limited spot. Interestingly, in addition, the FND also showed electron microscope (EM) contrast inside the cell. We hope that this FND will not only replace some of the common dyes used for SRM, but will also be used as a dual responsive marker in correlative super resolution microscopy (CLEM).

Graphical abstract: Dual responsive specifically labelled carbogenic fluorescent nanodots for super resolution and electron microscopy

Supplementary files

Article information

Article type
Communication
Submitted
15 Jan 2019
Accepted
19 Mar 2019
First published
20 Mar 2019

Nanoscale, 2019,11, 6561-6565

Dual responsive specifically labelled carbogenic fluorescent nanodots for super resolution and electron microscopy

Navneet. C. Verma, C. Rao, A. Singh, N. Garg and C. K. Nandi, Nanoscale, 2019, 11, 6561 DOI: 10.1039/C9NR00457B

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