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Visual detection of odorant geraniol enabled by integration of a human olfactory receptor into polydiacetylene/lipid nano-assembly

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Abstract

A new polydiacetylene lipid/human olfactory receptor nano-assembly was fabricated for the visual detection of an odorant for the first time. The assembly consisted of phospholipid-mixed polydiacetylenes (PDAs) and human olfactory receptors (hORs) in detergent micelles. To overcome the limitations of bioelectronic noses, hOR-embedded chromatic complexes (PDA/hORs) were developed, introducing PDAs that showed color and fluorescence transitions against various stimuli. The chromatic nanocomplexes reacted with target molecules, showing a fluorescence intensity increase in a dose-dependent manner and target selectivity among various odorants. As a result, a color transition of the assembly from blue to purple occurred, allowing the visual detection of the odorant geraniol. Through circular dichroism (CD) spectroscopy and a tryptophan fluorescence quenching method, the structural and functional properties of the hORs embedded in the complexes were confirmed. Based on this first work, future array devices, integrating multiple nano-assemblies, can be substantiated and utilized in environmental assessment and analysis of food quality.

Graphical abstract: Visual detection of odorant geraniol enabled by integration of a human olfactory receptor into polydiacetylene/lipid nano-assembly

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Publication details

The article was received on 09 Jan 2019, accepted on 21 Mar 2019 and first published on 29 Mar 2019


Article type: Communication
DOI: 10.1039/C9NR00249A
Citation: Nanoscale, 2019, Advance Article

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    Visual detection of odorant geraniol enabled by integration of a human olfactory receptor into polydiacetylene/lipid nano-assembly

    T. Kim, D. Moon, J. H. Park, H. Yang, S. Cho, T. H. Park and D. J. Ahn, Nanoscale, 2019, Advance Article , DOI: 10.1039/C9NR00249A

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