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Issue 8, 2019
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Preparation of a thickness-controlled Mg-MOFs-based magnetic graphene composite as a novel hydrophilic matrix for the effective identification of the glycopeptide in the human urine

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Abstract

The highly effective analysis of glycopeptides from complex biological samples is an attractive and critical topic all the time. In this study, a novel thickness-controlled hydrophilic Mg-metal organic frameworks (Mg-MOFs) coating-functionalized magnetic graphene composite (MagG@Mg-MOFs-1C) was prepared for the capture of the glycopeptides. The as-synthesized composite exhibits an ultralow limit of detection (0.1 fmol μL−1), a perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 500 : 500, w/w/w), and a high binding capacity (150 mg g−1), satisfying reusability and high recovery in the recognition of glycopeptides due to its outstanding characteristics including strong magnetic property, large surface area (617 m2 g−1), plenty of affinity sites, and excellent hydrophilicity. Furthermore, the MagG@Mg-MOFs-1C composite was successfully applied to selectively enriched glycopeptides in human urine. More excitingly, 406 N-glycosylation peptides corresponding to 185 glycoproteins were identified in the urine of the bladder cancer patients, in which these identified glycoproteins include the potential biomarkers (α-2-macroglobulin, complement C4-B, and α-1-antitrypsin) for the bladder cancer. This study suggests that the hydrophilic porous MOFs-functionalized composite has a great potential in the large-scale characterization of the low-abundance biomolecules in urine, opening a new avenue for the rapid and convenient diagnosis of the disease.

Graphical abstract: Preparation of a thickness-controlled Mg-MOFs-based magnetic graphene composite as a novel hydrophilic matrix for the effective identification of the glycopeptide in the human urine

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Publication details

The article was received on 13 Dec 2018, accepted on 22 Jan 2019 and first published on 24 Jan 2019


Article type: Paper
DOI: 10.1039/C8NR10074H
Citation: Nanoscale, 2019,11, 3701-3709

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    Preparation of a thickness-controlled Mg-MOFs-based magnetic graphene composite as a novel hydrophilic matrix for the effective identification of the glycopeptide in the human urine

    J. Wang, J. Li, G. Yan, M. Gao and X. Zhang, Nanoscale, 2019, 11, 3701
    DOI: 10.1039/C8NR10074H

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