Jump to main content
Jump to site search

Issue 22, 2019
Previous Article Next Article

Protein deglycosylation can drastically affect the cellular uptake

Author affiliations

Abstract

Targeted drug delivery mediated by nanocarriers is a major issue in modern-day medicine. Upon coming in contact with biological fluids (e.g. blood), nanocarriers are rapidly covered by biomolecules (proteins, lipids, etc.) which results in the formation of a surface layer, widely known as the biomolecular corona. The biomolecular corona subsequently confers a certain biological identity to the corona-covered nanocarriers which can be crucial during their subsequent interactions with cells or other biological entities. In contrast to the proteins of the corona, little is known about the impact of the non-protein constituents of the corona, such as sugars. Here, we investigate the role of protein glycosylation of the corona in cellular uptake. We show that deglycosylation of clusterin (CLU) and apolipoprotein AI (Apo AI) significantly changes (increases and decreases, respectively) the cellular uptake of nanocarriers covered with these proteins.

Graphical abstract: Protein deglycosylation can drastically affect the cellular uptake

Back to tab navigation

Supplementary files

Publication details

The article was received on 13 Oct 2018, accepted on 27 Apr 2019 and first published on 03 May 2019


Article type: Paper
DOI: 10.1039/C8NR08305C
Nanoscale, 2019,11, 10727-10737
  • Open access: Creative Commons BY license
  •   Request permissions

    Protein deglycosylation can drastically affect the cellular uptake

    A. Ghazaryan, K. Landfester and V. Mailänder, Nanoscale, 2019, 11, 10727
    DOI: 10.1039/C8NR08305C

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements