Protein deglycosylation can drastically effect the cellular uptake
Targeted drug delivery mediated by nanocarriers is a major issue in modern-day medicine. Upon coming in contact with biological fluids (e.g. blood), nanocarriers are rapidly covered by biomolecules (proteins, lipids etc.) which results in a formation of a surface layer, widely known as biomolecular corona. The biomolecular corona subsequently confers a certain biological identity to the corona-covered nanocarriers which can be crucial during subsequent interactions with cells or other biological entities. In contrast to the proteins of the corona, little is known about the impact of non-protein constituents to the corona, such as sugars. Here, we investigate the role of protein glycosylation of the corona for cellular uptake. We show that deglycosylation of clusterin (CLU) and apolipoprotein AI (Apo AI) significantly changes (increases and decreases, respectively) the cellular uptake of nanocarriers covered with those proteins.