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Fibrinogen-erythrocyte binding and hemorheology measurements on the assessment of essential arterial hypertension patients

Abstract

Some studies have reported a positive association between plasma fibrinogen levels, erythrocyte aggregation and essential arterial hypertension (EAH). The aim of this study was to understand how the interaction between fibrinogen and its erythrocyte membrane receptor are altered on EAH. EAH patients (n = 31) and healthy blood donors (n = 65) were enrolled in the study. EAH patients, were therapeutically controlled for the disease, presenting systolic blood pressure between 108 and 180 mm Hg and diastolic blood pressure between 66 and 123 mm Hg. Clinical evaluation included blood pressure monitoring, electrocardiography, echocardiography and blood cell count. The hemorheological parameters were also analyzed. Fibrinogen-erythrocyte binding force and frequency were evaluated quantitatively, at the single-molecule level, using atomic force microscopy (AFM). Changes on erythrocyte elasticity were also evaluated. Force spectroscopy data showed that the average fibrinogen-erythrocyte binding forces increase from 40.4 ± 3.0 pN in healthy donors to 73.8 ± 8.1 pN in patients with EAH, despite a lower binding frequency for patients, comparing to the control group (7.9 ± 1.6% vs. 27.6 ± 4.2%, respectively). Elasticity studies revealed an increase of erythrocyte stiffness on the patients. The stronger fibrinogen binding to erythrocytes from EAH patients and alteration on cells elasticity may lead to changes on the whole blood flow. The patients’ altered hemorheological parameters may also contribute for this blood flow perturbations. The transient bridging of two erythrocytes, by the simultaneous binding of fibrinogen to both of them, promoting erythrocyte aggregation, could represent an important cardiovascular risk factor.

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Publication details

The article was received on 30 May 2018, accepted on 06 Jan 2019 and first published on 08 Jan 2019


Article type: Paper
DOI: 10.1039/C8NR04398A
Citation: Nanoscale, 2019, Accepted Manuscript
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    Fibrinogen-erythrocyte binding and hemorheology measurements on the assessment of essential arterial hypertension patients

    A. F. Guedes, C. Moreira, J. B. Nogueira, N. C. Santos and F. A. Carvalho, Nanoscale, 2019, Accepted Manuscript , DOI: 10.1039/C8NR04398A

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