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CRISPR/Cas-based genome engineering in natural product discovery

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Abstract

Covering: up to February, 2018

This review briefly introduces and summarizes current knowledge about the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) – CRISPR/Cas system and how it was engineered to become one of the most important and versatile genome editing techniques that are currently revolutionizing the whole field of molecular biology. It aims to highlight and discuss the applications and remaining challenges of CRISPR/Cas (mainly focusing on CRISPR/SpCas9)-based genome editing in natural product discovery. The organisms covered include bacteria such as Streptomyces, Corynebacteria, and Myxobacteria; filamentous fungi such as Aspergillus, Beauveria, and Ganoderma; microalgae; and some plants. As closing remarks, the prospects of using CRISPR/Cas in natural product discovery will be discussed.

Graphical abstract: CRISPR/Cas-based genome engineering in natural product discovery

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Publication details

The article was received on 25 Oct 2018 and first published on 14 Dec 2018


Article type: Review Article
DOI: 10.1039/C8NP00089A
Citation: Nat. Prod. Rep., 2019, Advance Article
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    CRISPR/Cas-based genome engineering in natural product discovery

    Y. Tong, T. Weber and S. Y. Lee, Nat. Prod. Rep., 2019, Advance Article , DOI: 10.1039/C8NP00089A

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