Issue 46, 2019

One-pot synthesis of a recyclable ratiometric fluorescent probe based on MOFs for turn-on sensing of Mg2+ ions and bioimaging in live cells

Abstract

A novel recyclable ratiometric fluorescent probe based on MOFs was designed and synthesized by a one-step approach, which was realized by assembling Al-MOFs in an aqueous solution containing rhodamine B (RhB) as a fluorescent reagent and Fe3O4 as a magnetically recyclable reagent. The obtained fluorescent probe (Fe3O4/RhB@Al-MOFs) has two emissions under excitation at 330 nm, in which the emission at 440 nm is attributed to the Al-MOFs and the emission at 610 nm presumably originates from the RhB dye. It was noted that the probe displays responses to Mg2+ ions with fluorescence enhancement at 440 nm, whereas the fluorescence at 610 nm would be a constant reference fluorescence signal. The existence of RhB significantly improves the detection accuracy and broadens the linear range. As a result, the selectivity of Fe3O4/RhB@Al-MOFs towards Mg2+ compared to other metal ions is excellent. When the Mg2+ ions (262.5 ppm) are added, the ratio of fluorescence intensity (I440/I610) is changed from 2.47 to 11.5. The Fe3O4/RhB@Al-MOFs show a rapid detection process (90 s), high fluorescence enhancement (up to 5-fold) and a low detection limit of Mg2+ ions (8 × 10−7 M). In particular, the magnetic Fe3O4 endows Fe3O4/RhB@Al-MOFs with a high magnetic responsiveness, thus, facilitating the separation procedures. As expected, it can be easily separated and recycled without a significant loss of recognition performance after being used many times, and thus, exhibits good reusability. Meanwhile, the cell cytotoxicity experiments suggest that Fe3O4/RhB@Al-MOFs is nearly non-toxic and in vitro cell imaging experiments demonstrate that the probe is capable of successfully enhancing fluorescence from the intracellular area in HL-7702 and A375 cellular systems. Hence, it has the potential for the detection of Mg2+ ions inside biosystems.

Graphical abstract: One-pot synthesis of a recyclable ratiometric fluorescent probe based on MOFs for turn-on sensing of Mg2+ ions and bioimaging in live cells

Supplementary files

Article information

Article type
Paper
Submitted
03 Sep 2019
Accepted
25 Oct 2019
First published
28 Oct 2019

New J. Chem., 2019,43, 18377-18383

One-pot synthesis of a recyclable ratiometric fluorescent probe based on MOFs for turn-on sensing of Mg2+ ions and bioimaging in live cells

X. Gao, Y. Gao, R. Qi and L. Han, New J. Chem., 2019, 43, 18377 DOI: 10.1039/C9NJ04536H

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