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An efficient ruthenium tris(bipyridyl)-based chemosensor for the specific detection of cysteine and its luminescence imaging in living zebrafish

Abstract

A novel luminescent chemosensor based on , -unsaturated ketones conjugated Ru(bpy)32+ derivative (Ru-E) has been designed and synthesized for the highly selective and sensitive detection of cysteine in 100% aqueous solutions. Owing to the conjugate 1,4-addition of cysteine to , -unsaturated ketones, the non-luminescent chemosensor Ru-E enabled to specifically react with cysteine to form the corresponding product, Ru-Cys, accompanied by the remarkable luminescence enhancement at 620 nm. Job’s plot and mass spectrometry analysis indicate a 1:2 binding stoichiometry between Ru-E and Cys. The sensing mechanism is confirmed by NMR, mass and emission spectrometry. Moreover, Ru-E exhibited very low cytotoxicity and was successfully applied to image endogenous thiols both in living bone mesenchymal stem cells (BMSCs) and zebrafish. In addition, Ru-E showed a biological application for monitoring thiols in the newborn-calf serum. The results of this work demonstrate Ru-E would be a useful tool for physiological and pathological studies involving biothiols.

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Publication details

The article was received on 27 Aug 2019, accepted on 22 Oct 2019 and first published on 25 Oct 2019


Article type: Paper
DOI: 10.1039/C9NJ04426D
New J. Chem., 2019, Accepted Manuscript

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    An efficient ruthenium tris(bipyridyl)-based chemosensor for the specific detection of cysteine and its luminescence imaging in living zebrafish

    Y. Li, N. Shi and M. Li, New J. Chem., 2019, Accepted Manuscript , DOI: 10.1039/C9NJ04426D

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