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A method for SNP detection using MoS2@AuNPs and SYBR Green I in combination with enzyme digestion

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Abstract

Single-nucleotide polymorphisms (SNPs) in a gene sequence are markers for a variety of human diseases. SNP detection methods are time-consuming, need laboratory-scale settings, and are expensive. A simple, rapid and label-free method for detecting SNPs was developed in this study. SG (SYBR Green I), DNA sequences, and molybdenum disulfide–gold nanoparticles (MoS2@AuNPs) were used in this sensing system for a proof-of-principle study. DNA was immobilized on the surface of MoS2@AuNPs in order to remove the nonspecific probe displacement and false-positive signals using 10% Tween 80. MoS2@AuNPs quenched the fluorescence from SG intercalated into the DNA duplex. The SG fluorescence signals from the matched and mismatched DNA duplex were significantly different after the addition of MoS2. HapII can recognize the enzyme digestion site in the DNA sequence and further improve the effect of SNP detection. Therefore, this method can clearly distinguish SNPs in DNA sequences.

Graphical abstract: A method for SNP detection using MoS2@AuNPs and SYBR Green I in combination with enzyme digestion

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Publication details

The article was received on 21 Aug 2019, accepted on 21 Oct 2019 and first published on 23 Oct 2019


Article type: Paper
DOI: 10.1039/C9NJ04319E
New J. Chem., 2019, Advance Article

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    A method for SNP detection using MoS2@AuNPs and SYBR Green I in combination with enzyme digestion

    L. Yan, Z. Deng, H. Shi, B. Xie and L. Gao, New J. Chem., 2019, Advance Article , DOI: 10.1039/C9NJ04319E

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