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Positive charge-dependent cell targeted staining and DNA detection

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Abstract

The chemical structures or positive charge-guided specific cell organelles' staining was an interesting but very challenging research work. In this paper, we synthesized a series of triphenylamine (TPA) derivatives with mono-, di- and tri-pyridinium as substituent groups. By exchanging the negative ions (I, BF4 and PF6) between them, a platform containing small compounds was established. After that, mammalian cells of HeLa and plant cells of BY-2 were employed for the study on cell imaging. The results clearly demonstrated that mono-pyridinium derivatives (TPA-Py-I, TPA-Py-BF4 and TPA-Py-PF6) are mitochondrion-targeted (for HeLa cells) and cell wall-targeted (for BY-2 cells). However, both di- and tri-pyridinium substituted derivatives (TPA-diPy-BF4 and TPA-triPy-BF4) were nucleus-targeted for both cell lines. From these important findings, we can conclude that the quantity of positive charge plays a decisive role in different cell organelle-targeted staining. We also declared that our research results were very useful for designing and synthesizing probes with good cellular uptake capability, significantly enhanced targeting ability, and DNA detection.

Graphical abstract: Positive charge-dependent cell targeted staining and DNA detection

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Publication details

The article was received on 01 Aug 2019, accepted on 27 Oct 2019 and first published on 28 Oct 2019


Article type: Paper
DOI: 10.1039/C9NJ03993G
New J. Chem., 2019, Advance Article

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    Positive charge-dependent cell targeted staining and DNA detection

    P. Yin, T. Wang, Y. Yang, W. Yin, S. Zhang, Z. Yang, C. Qi and H. Ma, New J. Chem., 2019, Advance Article , DOI: 10.1039/C9NJ03993G

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