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Issue 33, 2019
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A label-free “turn-on” fluorescence platform for glucose based on AuNCs@MnO2 nanocomposites

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Abstract

Diabetes mellitus is a lifelong chronic metabolic disease; it poses a serious threat to human health. Blood glucose measurement is one of the most important tests for diagnosing diabetes. Therefore, establishing an accurate and reliable method for determining glucose levels in serum is still of great significance. In this work, a novel fluorescent sensing platform using gold nanoclusters (AuNCs) and MnO2 nanocomposites as fluorescent probes was designed for the detection of glucose. First, gold nanoclusters (Bromelain-AuNCs) were synthesized by using bromelain as a template. MnO2 nanosheets were added to the system, and stable AuNCs@MnO2 nanocomposites were formed in 5 min. The fluorescence was quenched due to the inner filter effect (IFE) and static quenching effect (SQE) in the system. However, in the presence of H2O2 produced by an enzymatic reaction of glucose oxidase (GOx), the MnO2 nanosheets are reduced to Mn2+ ions, releasing AuNCs. At this point, the fluorescence is restored in the system. Therefore, the glucose concentration can be indirectly detected from the H2O2 produced by the glucose oxidase enzymatic reaction. For glucose detection, the broad linear range of this method is 25 μmol L−1 to 30 mmol L−1 and the detection limit is 6.7 μmol L−1. The practical application of the system is demonstrated in human blood samples. Compared with the whole-blood automatic analyser, this method showed satisfactory results.

Graphical abstract: A label-free “turn-on” fluorescence platform for glucose based on AuNCs@MnO2 nanocomposites

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Publication details

The article was received on 18 Jun 2019, accepted on 22 Jul 2019 and first published on 24 Jul 2019


Article type: Paper
DOI: 10.1039/C9NJ03135A
New J. Chem., 2019,43, 13143-13151

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    A label-free “turn-on” fluorescence platform for glucose based on AuNCs@MnO2 nanocomposites

    S. Liu, C. Lv, R. Liu, G. Yang, S. Li, L. Zuo and P. Xue, New J. Chem., 2019, 43, 13143
    DOI: 10.1039/C9NJ03135A

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