Multifunctional dual-mesoporous silica nanoparticle loading protein and dual antitumor drugs as a targeted delivery system
A dual-mesoporous structure silica (2~4 nm and 4~16 nm) that modified with amino and carboxyl groups simultaneously was successfully synthesized. Doxorubicin (DOX) and cisplatin (Pt) were loaded into the interior of the spherical mesoporous silica carrier material and the macromolecular model drug bovine serum albumin (BSA) was loaded into the outer layer by using aminoguanidine-cyclodextrin as a blocking agent and introducing folic acid (FA) to achieve targeted functionalization, which constructing a multi-drug and protein therapy system with targeting functional and pH stimulation responsive controlled release system. Drug-loaded testing results showed that the amino and carboxyl functionalized nanoparticles display a high degree of drug loading of DOX, Pt and BSA. The loading rate of BSA reached 33%. In vitro drug release experiments confirmed that this drug carrier system could be achieve "zero pre-release". The cytotoxicity of dual-drug system is significantly higher than single-load drug delivery system and it reduces the toxicity of the drug itself for Hela cell. Multi-drug and protein therapy system with targeting functional showed a better synergistic targeted therapeutic effect for Hela cell. All experiments have shown that the combination of multi-drug and protein is a promising strategy.