High performance liquid chromatography associating with resonance Rayleigh scattering spectra study on the interactions of palladium (II)- antimigraine chelate with erythrosin B and their analytical applications
A highly sensitive and selectivity detection technology of high performance liquid chromatography associating with resonance Rayleigh scattering spectra (HPLC-RRS) is applied to analyze migraine drugs including zolmitriptan (ZON) and rizatriptan (RIN) for the first time. In HAc-NaAc buffer solution (pH = 4.5), ZON and palladium (II) chelate to form chelating cation [Pd•ZON]2+, and then react with Erythrosin B (Ery) via electrostatic attraction and hydrophobicity to form ternary complex [Pd•ZON]•Ery2, resulting in significantly enhanced RRS signal. The RRS was detected at the synchronous model. The calibration curves of ZON and RIN were linear in the range from 0.027 to 25 μg•mL-1, and the detection limit (S/N = 3) were 9.70 ng•mL-1 and 4.76 ng•mL-1 respectively. In addition, HPLC-RRS method showed that the recovery of the two migraine drugs was 98.90% to 100.87%, and the RSD (n = 6) was 1.67% to 4.86%. In this work, the experiment conditions were optimized, the reaction mechanism and the reason of RRS increment were studied. In human urine samples, the established method was applied to the synchronous detection of two antimigraine drugs with satisfactory results.