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Highly Biocompatible Zwitterionic Dendrimer-Encapsulated Platinum Nanoparticles for Sensitive Detection of Glucose in Complex Medium

Abstract

Development of ultra-small platinum nanoparticles is very important for sensitive detection of glucose in complex medium without protein interference. Zwitterionic dendrimer encapsulated platinum nanoparticles (Ptn-G5MC NPs) have been developed and studied for highly sensitive and selective colorimetric detection of glucose. Firstly, zwitterionic dendrimer (G5MC) was obtained by surface modification of generation 5 poly(amidoamine) dendrimer with maleic anhydride and cysteamine. The calculated average diameters of Pt nanoparticles (Pt NPs) for Ptn-G5MC (n = 55, 110, and 165) were 1.64 ± 0.22, 2.09 ± 0.24, and 2.98 ± 0.29 nm, respectively. Ptn-G5MC NPs not only had high stability in various pH solution and fibrinogen solution, but also showed no noticeable cytotoxicity against HUVEC cells and HeLa cells. In addition, Ptn-G5MC NPs catalyzed oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) with H2O2 to generate blue oxidized TMB (oxTMB), indicating their peroxidase-like property. This peroxidase-like reaction followed a typical Michaelis-Menten kinetics. Furthermore, this colorimetric reaction was used for glucose detection with high sensitivity and selectivity. The linear range of glucose concentration with absorbance at 652 nm was from 7 to 1380 μM, and the detection limit was 2.8 μM. More importantly, the relative activity of Ptn-G5MC NPs was not interfered by proteins. These enhanced properties are attributed to ultra-small size of Pt NPs and high stability of Pt55-G5MC NPs in complex medium. The glucose concentration in saliva as a real sample was 0.18 mM. The proposed glucose sensor could have promising applications for glucose detection in biocatalysis and bioassays in the future.

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Publication details

The article was received on 02 Mar 2019, accepted on 11 May 2019 and first published on 13 May 2019


Article type: Paper
DOI: 10.1039/C9NJ01101C
New J. Chem., 2019, Accepted Manuscript

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    Highly Biocompatible Zwitterionic Dendrimer-Encapsulated Platinum Nanoparticles for Sensitive Detection of Glucose in Complex Medium

    Y. Cui, J. Zhang, Q. Yu, X. Guo, S. Chen, H. Sun, S. Liu, L. Wang, X. Lai and D. Gao, New J. Chem., 2019, Accepted Manuscript , DOI: 10.1039/C9NJ01101C

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