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A novel near-infrared fluorescent hydrogen sulfide probe for live cell and tissue imaging

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Abstract

A dicyanoisophorone-based near-infrared fluorescent probe (NIR-NP) was successfully designed for hydrogen sulfide (H2S) detection. 7-Nitro-1,2,3-benzoxadiazole (NBD) amine, an electron-withdrawing group, was applied to quench the fluorescence of dicyanoisophorone-based fluorescent dyes and could be removed by H2S-specific thiolysis of an NBD amine bond, leading to a significant fluorescence turn-on response. The probe could quantitatively detect H2S with a lower detection limit (0.03 μM) in vitro and in living cells. The probe with a large Stokes shift (186 nm) showed a high selectivity to H2S in the presence of other biothiols, including glutathione, homocysteine, and cysteine. Moreover, the probe was successfully applied to image endogenous H2S in different tumor cells and in liver tissues. The excellent properties of the probe and its applications in cell and tissue imaging indicated its potential for further exploration and application of H2S in the pathophysiology of cancers and even in the diagnosis of H2S-related diseases.

Graphical abstract: A novel near-infrared fluorescent hydrogen sulfide probe for live cell and tissue imaging

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Publication details

The article was received on 13 Jan 2019, accepted on 11 Mar 2019 and first published on 19 Mar 2019


Article type: Paper
DOI: 10.1039/C9NJ00210C
Citation: New J. Chem., 2019, Advance Article

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    A novel near-infrared fluorescent hydrogen sulfide probe for live cell and tissue imaging

    X. Huang, H. Liu, J. Zhang, B. Xiao, F. Wu, Y. Zhang, Y. Tan and Y. Jiang, New J. Chem., 2019, Advance Article , DOI: 10.1039/C9NJ00210C

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