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Fluorescence sensing and intracellular imaging of Pd2+ ions by a novel coumarinyl-rhodamine Schiff base

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Abstract

Coumarinyl-rhodamine, HCR, served as an extremely selective sensor for Pd2+ ions in ethanol/H2O (8 : 2, v/v, HEPES buffer, pH 7.2) solution and the limit of detection (LOD) was 18.8 nM (3σ method). The free sensor, HCR, was weakly emissive and in the presence of Pd2+, the colour changed from straw to pink with very strong emission at 598 nm in the presence of eighteen other cations. A plausible mechanism involved opening of the spirolactam ring of rhodamine on interaction with Pd2+, which was justified by structure optimization and transition energy calculations using the DFT technique. HCR underwent 1 : 1 complexation with Pd2+, which was confirmed via the Job's plot, mass spectra and Benesi–Hildebrand plot (association constant Ka, 9.1 × 104 M−1). A separate in vitro experiment showed that HCR could specifically sense Pd2+ in MCF7 (human breast adenocarcinoma) cell lines.

Graphical abstract: Fluorescence sensing and intracellular imaging of Pd2+ ions by a novel coumarinyl-rhodamine Schiff base

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Publication details

The article was received on 26 Dec 2018, accepted on 26 Jan 2019 and first published on 29 Jan 2019


Article type: Paper
DOI: 10.1039/C8NJ06511J
Citation: New J. Chem., 2019, Advance Article

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    Fluorescence sensing and intracellular imaging of Pd2+ ions by a novel coumarinyl-rhodamine Schiff base

    A. K. Adak, R. Purkait, S. K. Manna, B. C. Ghosh, S. Pathak and C. Sinha, New J. Chem., 2019, Advance Article , DOI: 10.1039/C8NJ06511J

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