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Facile preparation of N,S-graphene oxide nanosheets as fluorescence “off-on” sensing platform for sensitive detection of biothiols

Abstract

Nitrogen and sulfur co-doped graphene oxide nanosheets (N,S-GO) is prepared via a facile one-step solvent-free approach by using citric acid as precursor and cysteine as dopant. Graphene nucleus is firstly formed via the intermolecular dehydration of citric acid; N and S are then incorporated into the graphene structure by attacking the margin of graphene nucleus. The cross-linking among the graphene nucleus via the intermolecular condensation leads to the final N,S-GO. The N,S-GO exhibits favorable photoluminescence behaviors such as high fluorescent quantum yield of 56.0%, excellent photoluminescence stability within the pH range of 5-12 and high tolerance to external ionic strength up to 1.0mol L-1 NaCl. The fluorescence energy transferring between AgNPs and N,S-GO can induce the efficient quenching of N,S-GO fluorescence, and the presence of biothiols in the system will restore the fluorescence effectively, thus a fluorescence “off-on” procedure is developed for the quantitative detection of biothiols. Under optimal conditions, the detection limit for cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) are 11.8nmol L-1, 4.9nmol L-1 and 7.3nmol L-1, respectively. The sensing platform exhibits good selectivity toward biothiols in the presence of other amino acids. The practicability of this sensing platform is well demonstrated by determining the biothiol content in human plasma samples, gaining the spiking recoveries of 97.10-101.50% with representative Cys.

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Publication details

The article was received on 28 Nov 2018, accepted on 03 Jan 2019 and first published on 04 Jan 2019


Article type: Paper
DOI: 10.1039/C8NJ06024J
Citation: New J. Chem., 2019, Accepted Manuscript
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    Facile preparation of N,S-graphene oxide nanosheets as fluorescence “off-on” sensing platform for sensitive detection of biothiols

    X. Zhao, X. Lin, J. Wang and X. Chen, New J. Chem., 2019, Accepted Manuscript , DOI: 10.1039/C8NJ06024J

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