Mitochondrial directed ratiometric fluorescent probe for quantitive detection of sulfur dioxide derivatives†
Abstract
We herein report a ratiometric fluorescent probe, MN, capable of determining SO32−/HSO3− in mitochondria. The probe specifically responds to SO32−/HSO3− and is not interfered with by other biological mercaptans or anions. The LOD of the probe is 85 nM, making it a feasible sensor to image SO32−/HSO3−. With good membrane permeability and low cytotoxicity, MN was successfully used in the ratiometric detection of SO32−/HSO3− in mitochondria.