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Encapsulation of collagenase within biomimetically mineralized metal–organic frameworks: designing biocomposites to prevent collagen degradation

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Abstract

A growing class of multifunctional porous materials termed metal–organic frameworks (MOFs) has attracted attention for immobilization of biomolecules via self-assembly of inorganic and organic building blocks. Herein, we report the rapid formation of MOF shells surrounding collagen-degrading enzymes by using a biomimetic mineralization approach. Bacterial collagenase, which is functionally related to endogenous proteases in the human body, was used as a nucleating agent to induce the mineralization of zeolitic imidazolate framework-8 (ZIF-8) as a crystalline shell. This zinc-based MOF material was selected due to its remarkable stability under physiological conditions and good biocompatibility. The straightforward, water-based synthesis yielded microporous collagenase-embedded ZIF-8 particles. Once immobilized inside the biomimetically mineralized MOF, the protease presented limited catalytic activity, being ineffective in the proteolysis of a collagen-like peptide. In conclusion, biomimetic mineralization of ZIF-8 using collagenase not only provided immobilization of the enzyme, but also enabled the control of its activity. This synthetic approach provides a potentially useful concept in the prevention of proteolytic activity involved in the degradation of collagen matrices in living organisms.

Graphical abstract: Encapsulation of collagenase within biomimetically mineralized metal–organic frameworks: designing biocomposites to prevent collagen degradation

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Publication details

The article was received on 15 Oct 2018, accepted on 04 Dec 2018 and first published on 04 Dec 2018


Article type: Paper
DOI: 10.1039/C8NJ05246H
Citation: New J. Chem., 2019, Advance Article
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    Encapsulation of collagenase within biomimetically mineralized metal–organic frameworks: designing biocomposites to prevent collagen degradation

    O. Bim Júnior, A. Bedran-Russo, J. B. S. Flor, A. F. S. Borges, V. F. Ximenes, R. C. G. Frem and P. N. Lisboa-Filho, New J. Chem., 2019, Advance Article , DOI: 10.1039/C8NJ05246H

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