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Issue 4, 2019
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An ESIPT based chromogenic and fluorescent ratiometric probe for Zn2+ with imaging in live cells and tissues

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Abstract

A new ESIPT based fluorescent probe has been introduced for selective detection of Zn2+ in human breast cancer cells. The designed probe exhibits a prominent ratiometric fluorescence change along with a sharp blue shift of ∼71 nm which can be attributed to the hampering of the ESIPT process. The probe is efficient enough to detect Zn2+ solely in the presence of other biologically relevant cations with a limit of detection value of 1.6 × 10−7 M. The experimentally observed changes in the structure and absorption properties of the probe after complete addition of Zn2+ were further studied by density functional theory (DFT) and time dependent density functional theory (TDDFT) calculations. Moreover, the dip-stick experiment with this probe displays both absorption and fluorescence color changes after exposure to Zn2+ which also turns out to be a potential application for the probe. Live cell imaging studies have been carried out using human breast cancer cells (MCF-7) and tissues.

Graphical abstract: An ESIPT based chromogenic and fluorescent ratiometric probe for Zn2+ with imaging in live cells and tissues

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Publication details

The article was received on 14 Sep 2018, accepted on 14 Dec 2018 and first published on 14 Dec 2018


Article type: Paper
DOI: 10.1039/C8NJ04695F
Citation: New J. Chem., 2019,43, 1857-1863
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    An ESIPT based chromogenic and fluorescent ratiometric probe for Zn2+ with imaging in live cells and tissues

    S. Gharami, K. Aich, D. Sarkar, P. Ghosh, N. Murmu and T. K. Mondal, New J. Chem., 2019, 43, 1857
    DOI: 10.1039/C8NJ04695F

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