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A simple yet effective AIE-based fluorescent nano-thermometer for temperature mapping in living cells using fluorescence lifetime imaging microscopy

Abstract

We designed and synthesized a novel nano-thermometer using Aggregation-Induced-Emission (AIE) molecules as the reporter and household butter as the matrix. This temperature nanosensor showed decreased fluorescence intensities (~2%/°C) and shorter fluorescence lifetime (~0.11 ns/°C) upon increasing the environmental temperature in the range of physiological temperature. Such fluorescence responses were reversible and independent of the environmental pH and ionic strength. The application of these nano-thermometer in temperature sensing in living cells using a fluorescence lifetime imaging microscopy (FLIM) was also demonstrated. To the best of our knowledge, this is the first example of AIE-based nano-thermometer for temperature sensing in living cells. This work also provides us with a simple, low-cost, and rapid fabrication method for an effective nanosensor design based on the AIE mechanism.

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Publication details

The article was received on 03 Nov 2019, accepted on 27 Nov 2019 and first published on 27 Nov 2019


Article type: Communication
DOI: 10.1039/C9NH00693A
Nanoscale Horiz., 2019, Accepted Manuscript
  • Open access: Creative Commons BY-NC license
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    A simple yet effective AIE-based fluorescent nano-thermometer for temperature mapping in living cells using fluorescence lifetime imaging microscopy

    H. Gao, C. Kam, T. Y. Chou, M. Wu, X. Zhao and S. Chen, Nanoscale Horiz., 2019, Accepted Manuscript , DOI: 10.1039/C9NH00693A

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