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Does conjugation strategy matter? Cetuximab-conjugated gold nanocages for targeting triple-negative breast cancer cells

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Abstract

The efficient targeting of cancer cells depends on the success of obtaining the active targeting of overexpressed receptors. A very accurate design of nanoconjugates should be done via the selection of the conjugation strategy to achieve effective targeted nanoconjugates. Here, we present a detailed study of cetuximab-conjugated nonspherical gold nanocages for the active targeting of triple-negative breast cancer cells, including MDA-MB-231 and MDA-MB-468. A few different general strategies were selected for monoclonal antibody conjugation to the nanoparticle surface. By varying the bioconjugation conditions, including antibody orientation or the presence of a polymeric spacer or recombinant protein biolinker, we demonstrate the importance of a rational design of nanoconjugates. A quantitative study of gold content via ICP-AES allowed us to compare the effectiveness of cellular uptake as a function of the conjugation strategy and confirmed the active nature of nanoparticle internalization in cancer cells via epidermal growth factor receptor recognition, corroborating the importance of the rational design of nanomaterials for nanomedicine.

Graphical abstract: Does conjugation strategy matter? Cetuximab-conjugated gold nanocages for targeting triple-negative breast cancer cells

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Publication details

The article was received on 12 Apr 2019, accepted on 22 Jul 2019 and first published on 23 Jul 2019


Article type: Paper
DOI: 10.1039/C9NA00241C
Nanoscale Adv., 2019, Advance Article
  • Open access: Creative Commons BY license
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    Does conjugation strategy matter? Cetuximab-conjugated gold nanocages for targeting triple-negative breast cancer cells

    S. Avvakumova, L. Pandolfi, E. Soprano, L. Moretto, M. Bellini, E. Galbiati, M. A. Rizzuto, M. Colombo, R. Allevi, F. Corsi, A. Sánchez Iglesias and D. Prosperi, Nanoscale Adv., 2019, Advance Article , DOI: 10.1039/C9NA00241C

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