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Carbon dots, a powerful non-toxic support for bioimaging by fluorescence nanoscopy and eradication of bacteria by photothermia

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Abstract

Carbon Dots (CDs) are innovative materials which have potential applications in many fields, including nanomedicine, energy and catalysis. Here CDs were produced by the alkali-assisted ultrasonic route and characterized by several techniques to determine their composition and properties. Fluorescence nanoscopy using single-molecule localization microscopy shows that they have very good photophysical properties and a remarkable blinking behaviour at 405 nm. Moreover, these CDs are a safe material, non-toxic towards different cell lines (cancer and non-cancer cells) even at very high concentration, reflecting an excellent biocompatibility. Photothermia, i.e. their heating capacity under laser irradiation, was evaluated at two wavelengths and at several power densities. The resulting temperature increment was high (5 < ΔT < 45 °C) and appropriate for biomedical applications. Bioimaging and photothermia were then performed on E. coli, a Gram(−) bacterium, incubated with CDs. Remarkably, by photothermia at 680 nm (0.3, 1 and 1.9 W cm−2) or 808 nm (1.9 W cm−2), CDs are able to eradicate bacteria in their exponential and stationary phases. Images obtained by 3D super-resolution microscopy clearly show the different CD distributions in surviving bacteria after mild photothermal treatment. These results confirm that CDs are multifunctional materials with a wide range of biomedical applications.

Graphical abstract: Carbon dots, a powerful non-toxic support for bioimaging by fluorescence nanoscopy and eradication of bacteria by photothermia

  • This article is part of the themed collection: Nanocarbons
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Publication details

The article was received on 05 Mar 2019, accepted on 17 May 2019 and first published on 17 May 2019


Article type: Paper
DOI: 10.1039/C9NA00140A
Nanoscale Adv., 2019, Advance Article
  • Open access: Creative Commons BY-NC license
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    Carbon dots, a powerful non-toxic support for bioimaging by fluorescence nanoscopy and eradication of bacteria by photothermia

    H. Belkahla, R. Boudjemaa, V. Caorsi, D. Pineau, A. Curcio, J. S. Lomas, P. Decorse, A. Chevillot-Biraud, T. Azaïs, C. Wilhelm, H. Randriamahazaka and M. Hémadi, Nanoscale Adv., 2019, Advance Article , DOI: 10.1039/C9NA00140A

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