Jump to main content
Jump to site search


Rutile nano-bio-interactions mediate dissimilar intracellular destiny in human skin cells

Abstract

The use of nanoparticles (NPs) in the healthcare market is growing exponentially, due to their unique physicochemical properties. Titanium dioxide nanoparticles (TiO2 NPs) are used in the formulation of sunscreens, due to their photoprotective capacity, but interactions of these particles with skin cells at the nanoscale are still unexplored. The present study was aimed to determine whether the initial nano-biological interactions, namely the formation of a nano-bio-complex (other than protein corona), can predict rutile internalization and intracellular trafficking in primary human fibroblasts and keratinocytes. Results show no significant effect of NPs on fibroblasts and keratinocytes viability, but cell proliferation was compromised due to nano-bio-interactions. The bio-complex formation is dependent upon the chemistry of the biological media, as well as NPs physicochemical properties, facilitating NPs internalization, and triggering autophagy in both cell types. For the first time, we observe that intracellular traffic of NPs is different when comparing the two skin cell models, and we have detected NPs within multivesicular bodies (MVBs) of keratinocytes. These structures grant the selected input of molecules involved in the biogenesis of exosomes, responsible for cells communication and, potentially, on structural equilibrium in human tissues. Nanoparticle-mediated alterations of the exosome quality, quantity and function can be another major source of nanotoxicity.

Back to tab navigation

Supplementary files

Publication details

The article was received on 08 Feb 2019, accepted on 10 Apr 2019 and first published on 12 Apr 2019


Article type: Paper
DOI: 10.1039/C9NA00078J
Citation: Nanoscale Adv., 2019, Accepted Manuscript
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Rutile nano-bio-interactions mediate dissimilar intracellular destiny in human skin cells

    P. Sanches, W. Souza, S. G. Piperni, A. L. Rossi, S. Scapin, V. Midlej, Y. Sade, A. P. Leme, M. Benchimol, L. A. Rocha, R. Bizon, R. Borojevic, J. M. Granjeiro and A. Ribeiro, Nanoscale Adv., 2019, Accepted Manuscript , DOI: 10.1039/C9NA00078J

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements