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Issue 3, 2019
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Facile construction of a DNA tetrahedron in unconventional ladder-like arrangements at room temperature

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Abstract

A DNA tetrahedron as the most classical and simplest three-dimensional DNA nanostructure has been widely utilized in biomedicine and biosensing. However, the existing assembly approaches usually require harsh thermal annealing conditions, involve the formation of unwanted by-products, and have poor size control. Herein, a facile strategy to fabricate a discrete DNA tetrahedron as a single, thermodynamically stable product in a quantitative yield at room temperature is reported. This system does not require a DNA trigger or thermal annealing treatment to initiate self-assembly. This DNA tetrahedron was made of three chemically ligated triangular-shaped DNAs in unconventional ladder-like arrangements, with measured heights of ∼4.16 ± 0.04 nm, showing extra protections for enzymatic degradation in biological environment. They show substantial cellular uptake in different cell lines via temperature, energy-dependent and clathrin-mediated endocytosis pathways. These characteristics allow our DNA tetrahedron to be used as vehicles for the delivery of very small and temperature-sensitive cargos. This novel assembly strategy developed for DNA tetrahedra could potentially be extended to other highly complex polyhedra; this indicated its generalizability.

Graphical abstract: Facile construction of a DNA tetrahedron in unconventional ladder-like arrangements at room temperature

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Publication details

The article was received on 01 Nov 2018, accepted on 23 Dec 2018 and first published on 27 Dec 2018


Article type: Paper
DOI: 10.1039/C8NA00323H
Citation: Nanoscale Adv., 2019,1, 1240-1248
  • Open access: Creative Commons BY license
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    Facile construction of a DNA tetrahedron in unconventional ladder-like arrangements at room temperature

    Z. Dai, H. M. Leung, Q. Gao, F. Wang, S. W. Wong, L. S. Liu, Y. J. Au, K. W. C. Lai and P. K. Lo, Nanoscale Adv., 2019, 1, 1240
    DOI: 10.1039/C8NA00323H

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