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A self-assembling peptide hydrogel for ultrarapid 3D bioassays

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Abstract

Biosensing analytical platforms rely on the intimate structure–function relationship of immobilized probes. In this context, hydrogels are appealing semi-wet systems to locally confine biomolecules while preserving their structural integrity and function. Yet, limitations imposed by biomolecule diffusion rates or fabrication difficulties still hamper their broad application. Here, using a self-assembling peptide, a printable and self-adhesive hydrogel was obtained and applied to fabricate arrays of localized bio-functional 3D microenvironments on analytical interfaces. This soft matrix represents a robust and versatile material, allowing fast and selective tuning of analyte diffusion, which is exploited here to run in-gel immunoassays under solution-like conditions in an unprecedented (<10 min) time frame. The developed material overcomes major limitations associated with hydrogels for bioassays, widening the prospects for easy fabrication of multifunctional bio-interfaces for high-throughput, molecular recognition assays.

Graphical abstract: A self-assembling peptide hydrogel for ultrarapid 3D bioassays

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Publication details

The article was received on 22 Aug 2018, accepted on 22 Oct 2018 and first published on 22 Oct 2018


Article type: Communication
DOI: 10.1039/C8NA00158H
Citation: Nanoscale Adv., 2019, Advance Article
  • Open access: Creative Commons BY-NC license
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    A self-assembling peptide hydrogel for ultrarapid 3D bioassays

    P. Gagni, A. Romanato, G. Bergamaschi, P. Bettotti, R. Vanna, C. Piotto, C. F. Morasso, M. Chiari, M. Cretich and A. Gori, Nanoscale Adv., 2019, Advance Article , DOI: 10.1039/C8NA00158H

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