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Photoinduced inhibition of DNA repair enzymes and the possible mechanism of photochemical transformations of the ruthenium nitrosyl complex [RuNO(β-Pic)2(NO2)2OH]

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Abstract

In this work we have demonstrated that the ruthenium nitrosyl complex [RuNO(β-Pic)2(NO2)2OH] is suitable for investigation of the inactivation of DNA repair enzymes in vitro. Photoinduced inhibition of DNA glycosylases such as E. coli Endo III, plant NtROS1, mammalian mNEIL1 and hNEIL2 occurs to an extent of ≥90% after irradiation with the ruthenium complex. The photophysical and photochemical processes of [RuNO(β-Pic)2(NO2)2OH] were investigated using stationary and time-resolved spectroscopy, and mass spectrometry. A possible mechanism of the photo-processes was proposed from the combined spectroscopic study and DTF calculations, which reveal that the photolysis is multistage. The primary and secondary photolysis stages are the photo-induced cleavage of the Ru–NO bond with the formation of a free nitric oxide and RuIII complex followed by ligand exchange with solvent. For E. coli Endo III, covalent interaction with the photolysis product was confirmed by UV-vis and mass spectrometric methods.

Graphical abstract: Photoinduced inhibition of DNA repair enzymes and the possible mechanism of photochemical transformations of the ruthenium nitrosyl complex [RuNO(β-Pic)2(NO2)2OH]

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Publication details

The article was received on 13 Jun 2019, accepted on 13 Sep 2019 and first published on 18 Sep 2019


Article type: Paper
DOI: 10.1039/C9MT00153K
Metallomics, 2019, Advance Article

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    Photoinduced inhibition of DNA repair enzymes and the possible mechanism of photochemical transformations of the ruthenium nitrosyl complex [RuNO(β-Pic)2(NO2)2OH]

    A. A. Mikhailov, D. V. Khantakova, V. A. Nichiporenko, E. M. Glebov, V. P. Grivin, V. F. Plyusnin, V. V. Yanshole, D. V. Petrova, G. A. Kostin and I. R. Grin, Metallomics, 2019, Advance Article , DOI: 10.1039/C9MT00153K

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