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Issue 8, 2019
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Study on the mechanism underlying Al-induced hepatotoxicity based on the identification of the Al-binding proteins in liver

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Abstract

Aluminum (Al) is the most abundant metal element in the earth's crust, and is implicated in the pathogenesis of liver lesions. However, the mechanisms underlying Al3+-induced hepatotoxicity are still largely elusive. Based on analysis with native gel electrophoresis, Al3+ plus 8-hydroxyquinoline staining and LC-MS/MS, the proteins with high Al3+ affinity were identified to be carbamoyl-phosphate synthase, adenosylhomocysteinase, heat shock protein 90-alpha, carbonic anhydrase 3, serum albumin and calreticulin. These proteins are involved in physiological processes such as the urea cycle, redox reactions, apoptosis and so on. Then we established an Al3+-treated rat model for biochemical tests, morphology observation and Ca2+ homeostasis analysis, in order to evaluate the extent of oxidative damage, hepatic histopathology and specific indicators of Al3+-related proteins in liver. Our findings indicated the high-affinity interactions with Al3+ perturbed the normal function of the above proteins, which could account for the mechanism underlying Al3+-induced hepatotoxicity.

Graphical abstract: Study on the mechanism underlying Al-induced hepatotoxicity based on the identification of the Al-binding proteins in liver

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Publication details

The article was received on 08 Jun 2019, accepted on 08 Jul 2019 and first published on 09 Jul 2019


Article type: Paper
DOI: 10.1039/C9MT00150F
Metallomics, 2019,11, 1353-1362

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    Study on the mechanism underlying Al-induced hepatotoxicity based on the identification of the Al-binding proteins in liver

    Y. Ding, J. Tang, X. You, X. Zhang, G. Wang, C. Yao, M. Lin, X. Wang and D. Cheng, Metallomics, 2019, 11, 1353
    DOI: 10.1039/C9MT00150F

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