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Selenium and tellurium-based nanoparticles as interfering factors on Quorum sensing-regulated processes: violacein production and bacterial biofilm formation

Abstract

A cell-to-cell communication system called quorum sensing (QS) promotes the transcription of certain target genes in bacterial cells leading to the activation of different cellular processes, some of them related to bacterial biofilm formation. The formation of bacterial biofilm favours antibiotic resistance that is nowadays a significant public-health problem. In this study, the effect of selenium (SeNPs) and tellurium (TeNPs) nanoparticles was examined in two bacterial processes mediated by QS: violacein production by Chromobacterium violaceum and biofilm formation by Pseudomonas aeruginosa. For this purpose, quantification of the pigment production in presence of these nanoparticles was monitored using the C. violaceum strain. Additionally, a combination of different microscopical imaging techniques was applied to examine the changes on the 3D biofilm structure of P. aeruginosa which were quantified through performing architectural metrics calculations (substratum area, cell area coverage and biovolume). SeNPs produces an 80% inhibition in the violacein production by C. violaceum and a significant effect in P. aeruginosa biofilm architecture (a reduction of 80% in the biovolume of the bacterial biofilm was obtained). TeNPs similarly affect violacein production and P. aeruginosa biofilm structure but at lower concentration levels. The results obtained suggest an important disruption of the QS signalling system by SeNPs and TeNPs supporting nanotechnology as a promising tool to fight against the emerging problem of bacterial resistance related to bacterial biofilm formation.

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Publication details

The article was received on 25 Feb 2019, accepted on 16 Apr 2019 and first published on 16 Apr 2019


Article type: Paper
DOI: 10.1039/C9MT00044E
Citation: Metallomics, 2019, Accepted Manuscript

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    Selenium and tellurium-based nanoparticles as interfering factors on Quorum sensing-regulated processes: violacein production and bacterial biofilm formation

    B. Gomez-Gomez, L. Arregui, S. Serrano, A. Santos, M. T. Perez-Corona and Y. Madrid, Metallomics, 2019, Accepted Manuscript , DOI: 10.1039/C9MT00044E

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