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Site directed mutagenesis of insulin-degrading enzyme allows singling out the molecular basis of peptidase versus E1-like activity: the role of metal ions

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Abstract

Four specifically designed IDE mutants have been used to unveil the molecular basis of peptidase versus E1-like activity of the enzyme. We have found that physiological concentrations of copper(II) ions inhibit the proteolytic activity of the enzyme towards small and large substrates but have no effect on the E1-like activity of the enzyme.

Graphical abstract: Site directed mutagenesis of insulin-degrading enzyme allows singling out the molecular basis of peptidase versus E1-like activity: the role of metal ions

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Publication details

The article was received on 12 Oct 2018, accepted on 20 Dec 2018 and first published on 20 Dec 2018


Article type: Communication
DOI: 10.1039/C8MT00288F
Citation: Metallomics, 2019, Advance Article
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    Site directed mutagenesis of insulin-degrading enzyme allows singling out the molecular basis of peptidase versus E1-like activity: the role of metal ions

    F. Bellia, V. Lanza, I. M. M. Ahmed, S. Garcia-Vinuales, E. Veiss, M. Arizzi, D. Calcagno, D. Milardi and G. Grasso, Metallomics, 2019, Advance Article , DOI: 10.1039/C8MT00288F

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