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Issue 7, 2019
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Synthesis of N-acyl amide natural products using a versatile adenylating biocatalyst

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Abstract

Natural products are secondary metabolites produced by many different organisms such as bacteria, fungi and plants. These biologically active molecules have been widely exploited for clinical application. Here we investigate TamA, a key enzyme from the biosynthetic pathway of tambjamine YP1, an acylated bipyrrole that is produced by the marine microorganism Pseudoalteromonas tunicata. TamA is a didomain enzyme composed of a catalytic adenylation (ANL) and an acyl carrier protein (ACP) domain that together control the fatty acid chain length of the YP1. Here we show that the TamA ANL domain alone can be used to generate a range of acyl adenylates that can be captured by a number of amines thus leading to the production of a series of fatty N-acyl amides. We exploit this biocatalytic promiscuity to produce the recently discovered class of N-acyl histidine amide natural products from Legionella pneumophila.

Graphical abstract: Synthesis of N-acyl amide natural products using a versatile adenylating biocatalyst

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Supplementary files

Article information


Submitted
01 Feb 2019
Accepted
22 May 2019
First published
31 May 2019

This article is Open Access

Med. Chem. Commun., 2019,10, 1192-1196
Article type
Research Article

Synthesis of N-acyl amide natural products using a versatile adenylating biocatalyst

P. M. Marchetti, S. M. Richardson, N. M. Kariem and D. J. Campopiano, Med. Chem. Commun., 2019, 10, 1192
DOI: 10.1039/C9MD00063A

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