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Issue 23, 2019
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Controllable cell manipulation in a microfluidic pipette-tip design using capacitive coupling of electric fields

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Abstract

Systems designed toward cell manipulation by electric fields are inherently challenged by energy dissipation along the electrode–electrolyte interface. A promising remedy is the introduction of high-k electrode passivation, enabling efficient capacitive coupling of electric fields into biological samples. We present the implementation of this strategy in a reusable pipette tip design featuring a 10 μl chamber volume for life science applications. Prototype validation and comparison to conductive gold-coated electrodes reveal a consistent and controllable biological effect that significantly increases the reproducibility of lysis events. The system provides precise descriptions of HEK-293 lysis dependency to variables such as field strength, frequency, and conductivity. Over 80% of cells were reversibly electroporated with minimal electrical lysis over a broad range of field settings. Successful transfection requires exponential decay pulses and showcases how modulating capacitive coupling can advance our understanding of fundamental mechanics in the field of electroporation.

Graphical abstract: Controllable cell manipulation in a microfluidic pipette-tip design using capacitive coupling of electric fields

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Publication details

The article was received on 18 Sep 2019, accepted on 20 Sep 2019 and first published on 31 Oct 2019


Article type: Paper
DOI: 10.1039/C9LC00927B
Lab Chip, 2019,19, 3997-4006
  • Open access: Creative Commons BY-NC license
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    Controllable cell manipulation in a microfluidic pipette-tip design using capacitive coupling of electric fields

    T. Wimberger, J. R. Peham, E. Ehmoser and K. J. Wassermann, Lab Chip, 2019, 19, 3997
    DOI: 10.1039/C9LC00927B

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