Jump to main content
Jump to site search


Injection molded open microfluidic well plate inserts for user-friendly coculture and microscopy

Author affiliations

Abstract

Open microfluidic cell culture systems are powerful tools for interrogating biological mechanisms. We have previously presented a microscale cell culture system, based on spontaneous capillary flow of biocompatible hydrogels, that is integrated into a standard cell culture well plate, with flexible cell compartment geometries and easy pipet access. Here, we present two new injection molded open microfluidic devices that also easily insert into standard cell culture well plates and standard culture workflows, allowing seamless adoption by biomedical researchers. These platforms allow culture and study of soluble factor communication among multiple cell types, and the microscale dimensions are well-suited for rare primary cells. Unique advances include optimized evaporation control within the well, manufacture with reproducible and cost-effective rapid injection molding, and compatibility with sample preparation workflows for high resolution microscopy (following well-established coverslip mounting procedures). In this work, we present several use cases that highlight the usability and widespread utility of our platform including culture of limited primary testis cells from surgical patients, microscopy readouts including immunocytochemistry and single molecule fluorescence in situ hybridization (smFISH), and coculture to study interactions between adipocytes and prostate cancer cells.

Graphical abstract: Injection molded open microfluidic well plate inserts for user-friendly coculture and microscopy

Back to tab navigation

Supplementary files

Publication details

The article was received on 19 Jul 2019, accepted on 15 Oct 2019 and first published on 12 Nov 2019


Article type: Paper
DOI: 10.1039/C9LC00706G
Lab Chip, 2020, Advance Article

  •   Request permissions

    Injection molded open microfluidic well plate inserts for user-friendly coculture and microscopy

    J. H. Day, T. M. Nicholson, X. Su, T. L. van Neel, I. Clinton, A. Kothandapani, J. Lee, M. H. Greenberg, J. K. Amory, T. J. Walsh, C. H. Muller, O. E. Franco, C. R. Jefcoate, S. E. Crawford, J. S. Jorgensen and A. B. Theberge, Lab Chip, 2020, Advance Article , DOI: 10.1039/C9LC00706G

Search articles by author

Spotlight

Advertisements