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Issue 6, 2019
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Paper-based multiplexed vertical flow assay for point-of-care testing

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We developed a multiplexed point-of-care immunodiagnostic assay for antibody detection in human sera made through the vertical stacking of functional paper layers. In this multiplexed vertical flow immunodiagnostic assay (xVFA), a colorimetric signal is generated by gold nanoparticles captured on a spatially-multiplexed sensing membrane containing specific antigens. The assay is completed in 20 minutes, following which the sensing membrane is imaged by a cost-effective mobile-phone reader. The images are sent to a server, where the results are rapidly analyzed and relayed back to the user. The performance of the assay was evaluated by measuring Lyme-specific antibodies in human sera as model target antibodies. The presented platform is rapid, simple, inexpensive, and allows for simultaneous and quantitative measurement of multiple antibodies and/or antigens making it a suitable point-of-care platform for disease diagnostics.

Graphical abstract: Paper-based multiplexed vertical flow assay for point-of-care testing

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Supplementary files

Article information

03 Jan 2019
31 Jan 2019
First published
31 Jan 2019

Lab Chip, 2019,19, 1027-1034
Article type
Author version available

Paper-based multiplexed vertical flow assay for point-of-care testing

H. Joung, Z. S. Ballard, A. Ma, D. K. Tseng, H. Teshome, S. Burakowski, O. B. Garner, D. Di Carlo and A. Ozcan, Lab Chip, 2019, 19, 1027
DOI: 10.1039/C9LC00011A

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