Jump to main content
Jump to site search

Issue 6, 2019
Previous Article Next Article

In situ visualization of hydrophilic spatial heterogeneity inside microfluidic chips by fluorescence microscopy

Author affiliations

Abstract

Fluorescence visualization for hydrophilic spatial heterogeneity inside microfluidic chips is a long-standing challenge owing to the lack of fluorescent dyes with high contrast between the target and the background noise. Herein, we used boronic acid in aggregation-induced emission (AIE) molecules as an anchor group towards modified hydroxyl groups, and an in situ visualization approach for hydrophilic spatial heterogeneity inside microfluidic chips was demonstrated. This success is based on the high-contrast of fluorescent behaviors for AIE molecules in aqueous solution and their immobilization by hydroxyl groups inside the microfluidic channels. In comparison to conventional laboratory-based ex situ techniques, the proposed strategy provides a direct representation for hydrophilic spatial heterogeneity, including the quantity and distribution of hydroxyl groups. This discovery not only identifies a previously unknown variability in hydrophilic spatial heterogeneity inside microfluidic channels, but also guides an optimal hydrophilic modification method in the channels.

Graphical abstract: In situ visualization of hydrophilic spatial heterogeneity inside microfluidic chips by fluorescence microscopy

Back to tab navigation

Supplementary files

Publication details

The article was received on 07 Dec 2018, accepted on 12 Feb 2019 and first published on 14 Feb 2019


Article type: Communication
DOI: 10.1039/C8LC01336E
Lab Chip, 2019,19, 934-940

  •   Request permissions

    In situ visualization of hydrophilic spatial heterogeneity inside microfluidic chips by fluorescence microscopy

    R. Tian, K. Li, W. Shi, C. Ding and C. Lu, Lab Chip, 2019, 19, 934
    DOI: 10.1039/C8LC01336E

Search articles by author

Spotlight

Advertisements