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Issue 3, 2019
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Fluorescence lifetime-activated droplet sorting in microfluidic chip systems

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Abstract

We present a highly efficient microfluidic fluorescence lifetime-activated droplet sorting (FLADS) approach as a novel technology for droplet manipulation in lab-on-a-chip devices. In a proof-of-concept study, we successfully applied the approach to sort droplets containing two different fluorescent compounds on the basis of their corresponding fluorescence lifetime. Towards this end, a technical set-up was developed enabling on-the-fly fluorescence lifetime determination of passing droplets. The herein developed LabVIEW program enabled fast triggering of a downstream dielectrophoretic force sorting functionality depending on average fluorescence lifetimes of individual droplets. The approach worked reliably at individual substrate concentrations from 1 nM to 1 mM. This not only allowed reliable sorting of droplets containing species with different fluorescence lifetimes but also enabled differentiation of mixtures in individual droplets.

Graphical abstract: Fluorescence lifetime-activated droplet sorting in microfluidic chip systems

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Supplementary files

Article information


Submitted
22 Nov 2018
Accepted
13 Dec 2018
First published
14 Dec 2018

Lab Chip, 2019,19, 403-409
Article type
Paper

Fluorescence lifetime-activated droplet sorting in microfluidic chip systems

S. Hasan, D. Geissler, K. Wink, A. Hagen, J. J. Heiland and D. Belder, Lab Chip, 2019, 19, 403
DOI: 10.1039/C8LC01278D

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