Jump to main content
Jump to site search


Droplet-based single cell RNAseq tools: a practical guide

Author affiliations

Abstract

Droplet based scRNA-seq systems such as Drop-seq, inDrop and Chromium 10X have been the catalyst for the wide adoption of high-throughput scRNA-seq technologies in the research laboratory. In order to understand the capabilities of these systems to deeply interrogate biology; here we provide a practical guide through all the steps involved in a typical scRNA-seq experiment. Through comparing and contrasting these three main droplet based systems (and their derivatives), we provide an overview of all critical considerations in obtaining high quality and biologically relevant data. We also discuss the limitations of these systems and how they fit into the emerging field of Genomic Cytometry.

Graphical abstract: Droplet-based single cell RNAseq tools: a practical guide

Back to tab navigation

Supplementary files

Publication details

The article was received on 11 Nov 2018, accepted on 04 Apr 2019 and first published on 08 Apr 2019


Article type: Perspective
DOI: 10.1039/C8LC01239C
Citation: Lab Chip, 2019, Advance Article

  •   Request permissions

    Droplet-based single cell RNAseq tools: a practical guide

    R. Salomon, D. Kaczorowski, F. Valdes-Mora, R. E. Nordon, A. Neild, N. Farbehi, N. Bartonicek and D. Gallego-Ortega, Lab Chip, 2019, Advance Article , DOI: 10.1039/C8LC01239C

Search articles by author

Spotlight

Advertisements