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Issue 10, 2019
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Droplet-based single cell RNAseq tools: a practical guide

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Droplet based scRNA-seq systems such as Drop-seq, inDrop and Chromium 10X have been the catalyst for the wide adoption of high-throughput scRNA-seq technologies in the research laboratory. In order to understand the capabilities of these systems to deeply interrogate biology; here we provide a practical guide through all the steps involved in a typical scRNA-seq experiment. Through comparing and contrasting these three main droplet based systems (and their derivatives), we provide an overview of all critical considerations in obtaining high quality and biologically relevant data. We also discuss the limitations of these systems and how they fit into the emerging field of Genomic Cytometry.

Graphical abstract: Droplet-based single cell RNAseq tools: a practical guide

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Publication details

The article was received on 11 Nov 2018, accepted on 04 Apr 2019 and first published on 08 Apr 2019

Article type: Perspective
DOI: 10.1039/C8LC01239C
Lab Chip, 2019,19, 1706-1727

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    Droplet-based single cell RNAseq tools: a practical guide

    R. Salomon, D. Kaczorowski, F. Valdes-Mora, R. E. Nordon, A. Neild, N. Farbehi, N. Bartonicek and D. Gallego-Ortega, Lab Chip, 2019, 19, 1706
    DOI: 10.1039/C8LC01239C

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